Selection of genetic agents from random peptide aptamer expression libraries.
نویسندگان
چکیده
منابع مشابه
The use of mRNA display to select high-affinity protein-binding peptides.
We report the use of "mRNA display," an in vitro selection technique, to identify peptide aptamers to a protein target. mRNA display allows for the preparation of polypeptide libraries with far greater complexity than is possible with phage display. Starting with a library of approximately 10(13) random peptides, 20 different aptamers to streptavidin were obtained, with dissociation constants a...
متن کاملPuzzling Peptides from a Phage Display Library
The commercial availability of random peptide libraries displayed on the M13 phage is increasing their use forstudies on epitope identification, enzyme inhibitors, receptor ligands, etc. In this study two experimentswhere planned for selection of peptides. First with sheep antibodies, the positive selector was IgG, preparedon Protein G column from a pool of 11 sheeps immunized...
متن کاملEmulsion PCR: A High Efficient Way of PCR Amplification of Random DNA Libraries in Aptamer Selection
Aptamers are short RNA or DNA oligonucleotides which can bind with different targets. Typically, they are selected from a large number of random DNA sequence libraries. The main strategy to obtain aptamers is systematic evolution of ligands by exponential enrichment (SELEX). Low efficiency is one of the limitations for conventional PCR amplification of random DNA sequence library in aptamer sel...
متن کاملGeneration of Aptamers from A Primer-Free Randomized ssDNA Library Using Magnetic-Assisted Rapid Aptamer Selection
Aptamers are oligonucleotides that can bind to specific target molecules. Most aptamers are generated using random libraries in the standard systematic evolution of ligands by exponential enrichment (SELEX). Each random library contains oligonucleotides with a randomized central region and two fixed primer regions at both ends. The fixed primer regions are necessary for amplifying target-bound ...
متن کاملYeast genetic selections to optimize RNA decoys for transcription factor NF- B
In vitro-selected RNA aptamers are potential inhibitors of diseaserelated proteins. Our laboratory previously isolated an RNA aptamer that binds with high affinity to human transcription factor NFB. This RNA aptamer competitively inhibits DNA binding by NFB in vitro and is recognized by its target protein in vivo in a yeast three-hybrid system. In the present study, yeast genetic selections wer...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Methods in enzymology
دوره 328 شماره
صفحات -
تاریخ انتشار 2000